Rosette-Like Arrangement of Polyoma Virus Particles in Infected Mouse-Embryo Cells
نویسندگان
چکیده
منابع مشابه
Extracellular self-assembly of virus-like particles from secreted recombinant polyoma virus major coat protein.
Mouse polyoma virus major coat protein (VP1) expressed from a recombinant baculovirus is efficiently transported to infected cell nuclei and assembles into protein nanospheres morphologically similar to natural capsids. The nanospheres readily combine with plasmid DNA to form a hybrid gene therapy agent known as virus-like particles (VLPs). To facilitate large-scale production of VLPs free from...
متن کاملPolynucleotide ligase activity in cells infected with simian virus 40, polyoma virus, or vaccinia virus.
The conversion of simian virus 40 (SV40) component II deoxyribonucleic acid to component I has been used to assay polynucleotide ligase in extracts of tissue culture cells. All cell types examined, including chicken, hamster, mouse, monkey, and human cells, contained adenosine triphosphate-dependent ligase. After infection of mouse embryo, monkey kidney, and HeLa cells with polyoma virus, SV40,...
متن کاملThe Effect of chick Embryonic Somites on Neural Rosette Formation in Mouse Embryonic Stem Cells
purpose: The aim of the present study is to understand if EBs can generate neural rosette upon co-culture with chick embryo somites. Materials and Methods: The mouse ES cells, line Royan Bl, were cultured in hanging drops to induce embryoid bodies (EBs) formation. Somites were isolated from the chick embryos and then embedded in alginate solution. Finally, alginate beads containing somites were...
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ژورنال
عنوان ژورنال: Journal of Bacteriology
سال: 1965
ISSN: 0021-9193,1098-5530
DOI: 10.1128/jb.89.2.535-536.1965